Article Abstract

Journal of Medical and Biological Science

Review|Published August 2020|Vol. 6 (4): 51-61.


Spirulina and Proportion of Changes in Blood Parameters of Rats (Rattus Norvegicus) in Growth

Mathieu Nahounou BLEYERE1*, Philippe Sansan KAMBOU2, Mohamado OUEDRAOGO1, Paul Angoué YAPO1

1Laboratory of Physiology, Pharmacology and Pharmacopoeia, Research Training-Unit of Sciences Nature, Nangui Abrogoua University, 02 PO Box 801 Abidjan 02, Côte d’Ivoire.

2Laboratory of Medical Biochemistry, Medical Sciences Research and Training Unit, Félix Houphouët Boigny University, Côte d’Ivoire.



To demonstrate the safety and active properties of Spirulina, a study was carried out in Wistar rats (Rattus norvegicus) which received in addition to the granulated industrial food, Spirulina as a supplement for three months. Four homogeneous groups of six rats each, three males and three females were formed. Group 1 (control) received conventional food (pellets FACI®) ad libitum and distilled water at 10 ml/kg body weight. As for groups 2, 3 and 4, they also received FACI® food, Spirulina at a rate of 10; 50; and 100 mg/kg body weight respectively. Blood samples performed at the orbital sinus were collected in 5 mL EDTA tubes and dry tubes. The collected blood in EDTA tubes was used to assay the blood count (CBC) when dry tubes were centrifuged at 3000 rpm for 10 min. The collected serum made it possible to dose the following biochemical parameters: AST, ALT, triglycerides, total cholesterol, bilirubin, renal markers and glucose. Analysis of obtained results indicated weight gain in treated animals. In terms of hematological parameters, no major disturbance of the hematopoietic system was observed over the entire study period. Results also revealed that transaminases, lipid and carbohydrate markers as well as renal and hepatic markers did not experience any disturbance which could affect the physiological functioning of animals at all doses. Likewise, different proteins presented levels revealing no abnormality. At the end of this study, it was noted that Spirulina supplementation acted favorably on biomarkers and could, therefore, contribute to improving the functioning of vital organs.

Keywords: Blood biomarkers, complementation, rat (Rattus norvegicus), Spirulina.

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