Nisin Production in a Two Liters Bioreactor Using Lactococcus lactis NCIM 2114
Sunita Singh, Dash Sukanta, Rinu Kooliyottil, Supradip Saha, Sangeeta Gupta, Sivanadane Mandjiny, Devang Upadhyay and Leonard Holmes
Research Paper I Published February,2016
Journal of Agricultural Science and Food Technology
ABSTRACT
The facultative anaerobe, Lactococcus lactis was
grown in batch fermentation (1.5 L working volume) at 30°C,
using 5% inoculums to initiate growth for the production of
the antimicrobial food additive nisin. L. lactis
strain NCIM 2114 produced a maximal nisin concentration of
50,400 IU/mL and 8,830 IU/mg biomass in MRS growth medium
using a 2 L bioreactor with optimal conditions of 25 rpm and
0.2 vvm determined by a Box Behnken design statistical
package. Nisin was obtained from cell-free extracts and
quantified using ultra-fast liquid chromatography (UFLC).
Maximum bioactive nisin concentration was found at 4 h and
harvested. Increasing aeration, agitation or time until
harvest had negative impact on bioactive product yield. In a
repeated trial under the same conditions, cell-free extracts
of nisin were measured by well diffusion assay at 240 IU/mL
(antimicrobial activity). Bioactive nisin was confirmed. The
optimal specific growth rate for L. lactis was
measured to be 0.25 h-1. This study shows
that active biomass production can be increased using
conditions determined by standard statistical methods.
Key Words: Nisin, Food
preservative, RP-UFLC, Fermentation technology, Bioreactor,
Bioactivity, Cell-free extract, Box Behnken.
- Journal of Agricultural Science and Food Technology (JASFT)
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- Research Journal of Educational Studies and Review (RJESR)
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- Research Journal of Engineering and Technology (RJET)
- Pearl Journal of Management, Social Science and Humanities (PJMSSH)